研究资料

The ART industry demands an innovative automated vitrification system for better quality control and standardization of labour-intensive procedures. The hydrogel-based system achieves a compact, high-throughput solution with efficacy similar to the conventional Cryotop method, marking a significant advancement in the automation of ART

在小鼠模型的体外受精（IVF）方案中，实验成功的核心取决于能否在体外有效复现精子获能过程。人输卵管液（HTF）培养基作为一种经广泛验证的通用型培养基，在众多应用场景中均能稳定实现优异的受精效果，但特定遗传背景的小鼠品系及特殊实验条件下，采用替代配方的培养基可能更具优势

本研究对 Biorocks ART 系列培养基进行了全面的性能验证。研究目标是评估 Biorocks KSOM、Biorocks HTF 及 Biorocks 矿物油联合使用时，对四种不同小鼠品系（B6D2F1、 ICR、KM、C57BL/6）经自然交配及体外受精获得的胚胎，其支持植入前胚胎发育的 能力。

小鼠胚胎体外培养是发育生物学、遗传学及辅助生殖技术的基石。体外受精（IVF） 的成功率高度依赖体外受精液和操作方法。本实验室前期已建立一套标准操作流程 （SOP），采用 Biorocks HTF 进行受精操作，并利用 Biorocks KSOM 进行受精后培养。 该培养体系旨在实现较高的胚胎发育率

The in vitro Culture of preimplantation mouse embryos is a fundamental technique underpinning advances in developmental biology, genetics, and assisted reproductive technologies [1]. The success of in vitro fertilization (IVF) is critically dependent on the composition and handling of the Culture media. Our laboratory has previously established a standard operating procedure (SOP) utilizing Biorocks Human Tubal Fluid (HTF) for fertilization and Biorocks Potassium Simplex Optimized Medium (KSOM) for post￾fertilization Culture, a system designed to provide high rates of embryonic development.

This study presents a comprehensive performance validation of the Biorocks ART media suite. Our objective was to evaluate the capacity of Biorocks KSOM, Biorocks HTF, and Biorocks Mineral Oil to collectively support preimplantation development across four different mouse strains (B6D2F1, ICR, KM, and C57BL/6) using embryos derived from both natural mating and IVF.